Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

Kazuo Kasahara; Toshiharu Saiki

doi:10.1117/1.3319569

1 January 2010 Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe

Kazuo Kasahara, Toshiharu Saiki

Author Affiliations +

Journal of Nanophotonics, Vol. 4, Issue 1, 043502 (January 2010). https://doi.org/10.1117/1.3319569

Abstract

Numerical simulation of fluorescence correlation spectroscopy (FCS) based on near-field scanning optical microscopy (NSOM) was performed. A finite-difference time-domain method was employed to calculate the electric field distribution in the vicinity of the NSOM aperture and the Brownian motion of nanoparticles was reproduced by a Monte Carlo simulation. The physical validity of the simulation result was confirmed by comparing it with a theoretical calculation using a Gaussian observation volume, which is applied to FCS based on conventional optical microscopy.

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Kazuo Kasahara and Toshiharu Saiki "Numerical simulation of near-field fluorescence correlation spectroscopy using a fiber probe," Journal of Nanophotonics 4(1), 043502 (1 January 2010). https://doi.org/10.1117/1.3319569

Published: 1 January 2010

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