Special Section on Nanostructured Thin Films: From Theoretical Aspects to Practical Applications

Effect of surface functionalization of porous silicon microcavities on biosensing performance

[+] Author Affiliations
Marta Martin, Thierry Cloitre

Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Laurent Massif, Elias Estephan

Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

CNRS, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Université Montpellier 1, EA 4203, UFR Odontologie, 34193 Montpellier Cedex 5, France

Maribelle Saab, Csilla Gergely

Université Montpellier 2, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

CNRS, Laboratoire Charles Coulomb UMR 5221, F-34095, Montpellier, France

Christian Larroque

Université Montpellier 1, CRLC Val d’Aurelle-Paul Lamarque, IRCM, 34298 Montpellier, France

Vivechana Agarwal

CIICAP-UAEM, Av. Universidad 1001, Col Chamilpa, Cuernavaca, Mor., México

Frederic J. G. Cuisinier

Université Montpellier 1, EA 4203, UFR Odontologie, 34193 Montpellier Cedex 5, France

Guy Le Lay

CINAM-CNRS, Campus de Luminy, Case 913, 13288 Marseille cedex, France

Université de Provence, Marseille, France

J. Nanophoton. 6(1), 061506 (May 10, 2012). doi:10.1117/1.JNP.6.061506
History: Received December 12, 2011; Revised March 19, 2012; Accepted March 28, 2012
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Abstract.  Surface functionalization methods were investigated for their effects on the sensing performances of porous silicon (p-Si) microcavities when used for detection of biomolecules. These microcavities were fabricated to reveal reflectivity pass-band spectra in the visible and near-infrared spectral regime. In one approach, the devices were thermally oxidized and functionalized to ensure covalent binding of molecules. In the second approach, the as-etched p-Si surface was modified with adhesion peptides, isolated via phage display, that present high binding capacity for silicon. Functionalization and molecular binding events were monitored via reflectometric interference spectra as shifts in the resonance peaks of the cavity structure due to changes in the refractive index when a biomolecule is attached to the large internal surface of p-Si. Improved sensitivity was obtained owing to the peptide interface linkers between the p-Si and biological molecules compared to the silanized devices. Investigating the formation of peptide–Si interface layer via X-ray photoelectron spectroscopy, scanning tunneling microscopy, and scanning electron microscopy, we found that peptides form nanometer-thin layers on the Si surface and that their binding energy depends on the sequence of the peptide.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Topics

Silicon

Citation

Marta Martin ; Laurent Massif ; Elias Estephan ; Maribelle Saab ; Thierry Cloitre, et al.
"Effect of surface functionalization of porous silicon microcavities on biosensing performance", J. Nanophoton. 6(1), 061506 (May 10, 2012). ; http://dx.doi.org/10.1117/1.JNP.6.061506


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