Special Section on Fundamental and Applied Nanoelectromagnetics

Plasmon-enhanced fluorescence of labeled biomolecules on top of a silver sol-gel film

[+] Author Affiliations
Svetlana V. Vaschenko

Belarusian State University, The Chemical Department, Nezavisimosti Avenue 4, 220030 Minsk, Belarus

National Academy of Sciences of Belarus, B.I. Stepanov Institute of Physics, Nezavisimosti Avenue 68, 220072 Minsk, Belarus

Andrei A. Ramanenka

Belarusian State University, The Physical Department, Nezavisimosti Avenue 4, 220030 Minsk, Belarus

Dmitry V. Guzatov

Yanka Kupala Grodno State University, Ozheshko Street 22, 230023 Grodno, Belarus

Vyacheslav V. Stankevich

National Academy of Sciences of Belarus, B.I. Stepanov Institute of Physics, Nezavisimosti Avenue 68, 220072 Minsk, Belarus

Anatoly Ya. Lunevich

ELTA Ltd., Aviatsionnaya Street 19/6, 123182 Moscow, Russia

Yuri F. Glukhov

ELTA Ltd., Aviatsionnaya Street 19/6, 123182 Moscow, Russia

Iosif F. Sveklo

Yanka Kupala Grodno State University, Ozheshko Street 22, 230023 Grodno, Belarus

University of Bialystok, Faculty of Physics, 41 Lipowa Street, 15-424 Bialystok, Poland

Sergey V. Gaponenko

National Academy of Sciences of Belarus, B.I. Stepanov Institute of Physics, Nezavisimosti Avenue 68, 220072 Minsk, Belarus

J. Nanophoton. 6(1), 061710 (Oct 16, 2012). doi:10.1117/1.JNP.6.061710
History: Received June 29, 2012; Revised September 24, 2012; Accepted September 25, 2012
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Abstract.  A simple chemical technique was devised for the fabrication of silver nanostructured substrates which can be used for plasmonic enhancement of labeled proteins fluorescence. For bovine serum albumin labeled with fluorescein isothiocyanate, the obtained enhancement factor ranges from three to seven, depending on metal-luminophore spacing and silver nanoparticle size. For excitation with linear polarized light, the enhancement factor increases noticeably for p-polarization and decreases for s-polarization. The experimental results were interpreted in terms of the theoretical model in which the enhancement factor depends on incident light polarization, luminophoremetal spacing and silver nanoparticle size. Proposed plasmonic substrates can be considered as an affordable replacement of standard ones in different types of fluorescent assays for the purpose of increasing sensitivity.

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© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Svetlana V. Vaschenko ; Andrei A. Ramanenka ; Dmitry V. Guzatov ; Vyacheslav V. Stankevich ; Anatoly Ya. Lunevich, et al.
"Plasmon-enhanced fluorescence of labeled biomolecules on top of a silver sol-gel film", J. Nanophoton. 6(1), 061710 (Oct 16, 2012). ; http://dx.doi.org/10.1117/1.JNP.6.061710


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