Special Section on Nanostructured Thin Films: Fundamentals and Applications

Nanotopography of collagen thin films in correlation with fibroblast response

[+] Author Affiliations
Andreas Stylianou

National Technical University of Athens, School of Electrical and Computer Engineering, Biomedical Optics and Applied Biophysics Laboratory, Iroon Polytechniou 9, Athens 15780, Greece

Dido Yova

National Technical University of Athens, School of Electrical and Computer Engineering, Biomedical Optics and Applied Biophysics Laboratory, Iroon Polytechniou 9, Athens 15780, Greece

Eleni Alexandratou

National Technical University of Athens, School of Electrical and Computer Engineering, Biomedical Optics and Applied Biophysics Laboratory, Iroon Polytechniou 9, Athens 15780, Greece

J. Nanophoton. 7(1), 073590 (Jun 25, 2013). doi:10.1117/1.JNP.7.073590
History: Received December 15, 2012; Revised May 29, 2013; Accepted May 31, 2013
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Abstract.  Collagen thin films consisting of randomly oriented and oriented collagen fibrils/fibers are fabricated by hydrodynamic flow and spin coating, and then they are characterized by atomic force microscopy (AFM). Fibroblasts are cultured on these films in order to correlate their morphology and alignment, which are assessed with fluorescence and AFM imaging with different film characteristics. The results showed that the formed films could be used as substrates for culturing cells. Furthermore, cells reacted to film nanocharacteristics and especially to the orientation of fibrils/fibers. The investigation of the influence that the substrate nanotopography has on cells will help to elucidate the mechanisms of cell–biomaterial interactions, and will enable the design of intelligent coatings for implants and tissue engineering purposes.

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© 2013 Society of Photo-Optical Instrumentation Engineers

Topics

Collagen ; Thin films

Citation

Andreas Stylianou ; Dido Yova and Eleni Alexandratou
"Nanotopography of collagen thin films in correlation with fibroblast response", J. Nanophoton. 7(1), 073590 (Jun 25, 2013). ; http://dx.doi.org/10.1117/1.JNP.7.073590


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