Special Section on Nanobiophotonics and Related Techniques

Mouse neuroblastoma cell-based model and the effect of epileptic events on calcium oscillations and neural spikes

[+] Author Affiliations
Suhwan Kim

Kyungpook National University, School of Electrical Engineering and Computer Science, Daegu 702-701, Republic of Korea

Unsang Jung

Kyungpook National University, School of Electrical Engineering and Computer Science, Daegu 702-701, Republic of Korea

Juyoung Baek

Kyungpook National University, School of Electrical Engineering and Computer Science, Daegu 702-701, Republic of Korea

Sangwon Lee

Kyungpook National University, School of Electrical Engineering and Computer Science, Daegu 702-701, Republic of Korea

Woonggyu Jung

Ulsan National Institute of Science and Technology, School of Nano-Bioscience and Chemical Engineering, UNIST-gil 50(100 Banyeon-ri), Eonyang-eup, Ulju-gun, Ulsan Metropolitan City 689-798, Republic of Korea

Jeehyun Kim

Kyungpook National University, College of IT Engineering, School of Electronics Engineering, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea

Shinwon Kang

Kyungpook National University, College of IT Engineering, School of Electronics Engineering, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea

J. Nanophoton. 7(1), 073794 (Nov 05, 2013). doi:10.1117/1.JNP.7.073794
History: Received July 18, 2013; Revised September 26, 2013; Accepted October 3, 2013
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Abstract.  Recently, mouse neuroblastoma cells have been considered as an attractive model for the study of human neurological and prion diseases, and they have been intensively used as a model system in different areas. For example, the differentiation of neuro2a (N2A) cells, receptor-mediated ion current, and glutamate-induced physiological responses have been actively investigated with these cells. These mouse neuroblastoma N2A cells are of interest because they grow faster than other cells of neural origin and have a number of other advantages. The calcium oscillations and neural spikes of mouse neuroblastoma N2A cells in epileptic conditions are evaluated. Based on our observations of neural spikes in these cells with our proposed imaging modality, we reported that they can be an important model in epileptic activity studies. We concluded that mouse neuroblastoma N2A cells produce epileptic spikes in vitro in the same way as those produced by neurons or astrocytes. This evidence suggests that increased levels of neurotransmitter release due to the enhancement of free calcium from 4-aminopyridine causes the mouse neuroblastoma N2A cells to produce epileptic spikes and calcium oscillations.

Figures in this Article
© 2013 Society of Photo-Optical Instrumentation Engineers

Topics

Calcium ; Epilepsy

Citation

Suhwan Kim ; Unsang Jung ; Juyoung Baek ; Sangwon Lee ; Woonggyu Jung, et al.
"Mouse neuroblastoma cell-based model and the effect of epileptic events on calcium oscillations and neural spikes", J. Nanophoton. 7(1), 073794 (Nov 05, 2013). ; http://dx.doi.org/10.1117/1.JNP.7.073794


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