Research Papers

Development of high-sensitive, reproducible colloidal surface-enhanced Raman spectroscopy active substrate using silver nanocubes for potential biosensing applications

[+] Author Affiliations
Kudilatt Hasna

Cochin University of Science and Technology, Department of Physics, Nanophotonic and Optoelectronic Devices Laboratory, Kochi 682 022, India

Cochin University of Science and Technology, Department of Instrumentation, Kochi 682 022, India

Kiran Lakshmi

Cochin University of Science and Technology, Department of Biotechnology, Plant Biotechnology Laboratory, Kochi 682 022, India

Madambi Kunjukuttan Ezhuthachan Jayaraj

Cochin University of Science and Technology, Department of Physics, Nanophotonic and Optoelectronic Devices Laboratory, Kochi 682 022, India

Kumaran Rajeev Kumar

Cochin University of Science and Technology, Department of Instrumentation, Kochi 682 022, India

Murukeshan Vadakke Matham

Nanyang Technological University, School of Mechanical and Aerospace Engineering, The Centre for Optical and Laser Engineering, 50 Nanyang Avenue, Singapore 639798, Singapore

J. Nanophoton. 10(2), 026020 (May 23, 2016). doi:10.1117/1.JNP.10.026020
History: Received February 3, 2016; Accepted May 2, 2016
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Abstract.  Surface-enhanced Raman spectroscopy (SERS) has emerged as one of the thrust research areas that could find potential applications in bio and chemical sensing. We developed colloidal SERS active substrate with excellent sensitivity and high reproducibility using silver nanocube (AgNC) synthesized via the solvothermal method. Finite-difference time-domain simulation was carried out in detail to visualize dipole generation in the nanocube during localized surface plasmon resonance and to locate the respective hot spots in AgNC responsible for the huge Raman enhancement. The prediction is verified by the SERS analysis of the synthesized nanocubes using Rhodamine 6G molecule. An excellent sensitivity with a detection limit of 1017  M and a very high enhancement factor of 1.2×108 confirms the “hot spots” in the nanocube. SERS activity is also carried out for crystal violet and for food adulterant Sudan I molecule. Finally, label-free DNA detection is performed to demonstrate the versatility of SERS as a potential biosensor.

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© 2016 Society of Photo-Optical Instrumentation Engineers

Citation

Kudilatt Hasna ; Kiran Lakshmi ; Madambi Kunjukuttan Ezhuthachan Jayaraj ; Kumaran Rajeev Kumar and Murukeshan Vadakke Matham
"Development of high-sensitive, reproducible colloidal surface-enhanced Raman spectroscopy active substrate using silver nanocubes for potential biosensing applications", J. Nanophoton. 10(2), 026020 (May 23, 2016). ; http://dx.doi.org/10.1117/1.JNP.10.026020


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