Plasmon-based determination of macromolecular interactions with membrane-encapsulated nanoparticles

Joanna L. Richens; Jonathan P. Bramble; Jennifer Bain; Paul O'Shea

doi:10.1117/12.2306974

15 May 2018 Plasmon-based determination of macromolecular interactions with membrane-encapsulated nanoparticles

Joanna L. Richens, Jonathan P. Bramble, Jennifer Bain, Paul O'Shea

Proceedings Volume 10672, Nanophotonics VII; 1067208 (2018) https://doi.org/10.1117/12.2306974
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Nanoparticles exhibit various optical properties arising from scattering and absorption due to polariton excitation. The resulting frequency and amplitude is dependent on several factors such as particle size, shape, and dielectric environment. By modifying the environment of the nanoparticle surface, in particular by encapsulating an individual nanoparticle within a membrane bilayer comprising defined phospholipids, these properties may be utilised to interrogate molecular interactions adjacent to the particle surface to useful levels of sensitivity. We describe the underlying rationale of these properties and characterise the preparation and behaviour of the nanoparticles. We indicate the potential this approach may have for sensing and screening in analytical biomolecular technology by demonstrating that it can be utilised to reveal the kinetics of the molecular interactions of membrane associated events. We also indicate that the technique may yield higherorder structural information of the macromolecule-membrane interactions in a highly sensitive manner and discuss the physical origins of these potentially more exotic phenomena.

Conference Presentation

Citation Download Citation

Joanna L. Richens, Jonathan P. Bramble, Jennifer Bain, and Paul O'Shea "Plasmon-based determination of macromolecular interactions with membrane-encapsulated nanoparticles", Proc. SPIE 10672, Nanophotonics VII, 1067208 (15 May 2018); https://doi.org/10.1117/12.2306974

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available