Quantitative analysis of ceftazidime using SERS based on silver nanoparticles substrate

Haiyan Qi; Huacai Chen; Shuhui Zhao; Jie Huang

doi:10.1117/12.2327048

25 October 2018 Quantitative analysis of ceftazidime using SERS based on silver nanoparticles substrate

Haiyan Qi, Huacai Chen, Shuhui Zhao, Jie Huang

Proceedings Volume 10823, Nanophotonics and Micro/Nano Optics IV; 1082313 (2018) https://doi.org/10.1117/12.2327048
Event: SPIE/COS Photonics Asia, 2018, Beijing, China

Abstract

With the increasing abuse of antibiotics, more attention has been focused on the potential harm on ecological environment and human health. The aim of this study was quantitative analysis of ceftazidime using Surface Enhanced Raman spectroscopy (SERS). Flower-shaped silver nanoparticles adsorbed on silicon wafer were fabricated in an aqueous medium without heavy metal or organic wastes. The limits of detection (LOD) of Rhodamine 6G (R6G) could reached to 10^-9 M, indicating that the substrates had high SERS activity. Meanwhile, the substrates showed excellent stability and uniformity. Flower-shaped silver nanoparticles substrates were selected as substrates for detecting the SERS spectra of ceftazidime in different concentrations. The information about the structure of ceftazidime molecule was reflect by Raman vibration assignments efficiently. Based on the Raman characteristic bands of ceftazidime, four quantitative analysis models using linear regression were compared. It was found that equation between log₁₀C (C refers to the concentration of ceftazidime) and Iavg (average intensity) of Raman characteristic bands (749 cm^-1 , 850 cm^-1 and 1025 cm^-1) was more suitable for quantitative analysis of ceftazidime, and correlation coefficient R² was up to 0.96. The quantitative model was used to detect the concentration of ceftazidime in practical surface water sample (10^-2 g/L), and the relative error between actual values and calculated values was 1.95%. This method is high sensitivity and rapid, which is potentially a powerful tool for quantitative analysis of other antibiotics.

Citation Download Citation

Haiyan Qi, Huacai Chen, Shuhui Zhao, and Jie Huang "Quantitative analysis of ceftazidime using SERS based on silver nanoparticles substrate", Proc. SPIE 10823, Nanophotonics and Micro/Nano Optics IV, 1082313 (25 October 2018); https://doi.org/10.1117/12.2327048

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Silver

Nanoparticles

Surface enhanced Raman spectroscopy

Raman spectroscopy

Quantitative analysis

Silicon

Molecules

Show All Keywords

Keywords/Phrases

Search In:

Publication Years