Quantitative absorption imaging of single nanoparticles by widefield interferometric photothermal microscopy

Yu-Chien Huang; Te-Hsin Chen; Jz-Yuan Juo; Shi-Wei Chu; Chia-Lung Hsieh

doi:10.1117/12.2615856

27 October 2021 Quantitative absorption imaging of single nanoparticles by widefield interferometric photothermal microscopy

Yu-Chien Huang, Te-Hsin Chen, Jz-Yuan Juo, Shi-Wei Chu, Chia-Lung Hsieh

Author Affiliations +

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 1192518 (2021) https://doi.org/10.1117/12.2615856
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

We demonstrate widefield interferometric photothermal microscopy to detect the local refractive index changes of single nanoparticles induced by the dissipated heat due to light absorption (known as the thermal lens effect). The sensitivity of our system is sufficient for visualizing very small single gold nanoparticles, as small as 5 nm, with a dissipated heat of a few nanowatts. We establish a model to explain the generation mechanism of the photothermal signal. For small particles (< 40 nm), the photothermal signal is contributed by the scattered field of the thermal lens, independent of the particle size and materials. For large particles, we find that the strong scattered field of the sample would interfere with that of the thermal lens, leading to an enhancement of the photothermal contrast. This work shows that the complete scattering property of the sample is required for accurate absorption quantification through photothermal measurement.

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Yu-Chien Huang, Te-Hsin Chen, Jz-Yuan Juo, Shi-Wei Chu, and Chia-Lung Hsieh "Quantitative absorption imaging of single nanoparticles by widefield interferometric photothermal microscopy", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 1192518 (27 October 2021); https://doi.org/10.1117/12.2615856

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KEYWORDS

Nanoparticles

Microscopy

Absorption

Particles

Interferometry

Scattering

Light scattering

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