Distribution of photosensitizers between tumor cells and tumor infiltrating host cells

Mladen Korbelik; Gorazd Krosl

doi:10.1117/12.168679

1 March 1994 Distribution of photosensitizers between tumor cells and tumor infiltrating host cells

Mladen Korbelik, Gorazd Krosl

Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168679
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Photofrin levels in different cellular populations constituting a murine FsaR fibrosarcoma were measured by flow cytometry. Both myeloid and lymphoid populations associated with the tumor were found to accumulate more photosensitizer on a per cell basis, on average, than the malignant cells. Macrophages, identified by the F4/80 antigen, exceeded other myeloid cells in Photofrin accumulation. It is shown that one of the factors involved is the variability in the photosensitizer content in cells located at different distances from the nearest blood vessel. This was investigated by a flow cytometry technique with the fluorescent stain Hoechst 33342, used to distinguish cells depending on their proximity to the tumor vasculature.

Citation Download Citation

Mladen Korbelik and Gorazd Krosl "Distribution of photosensitizers between tumor cells and tumor infiltrating host cells", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); https://doi.org/10.1117/12.168679

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