Detection of fluorophore-labeled antibodies by surface-enhanced fluorescence on metal nanoislands

Thomas G. M. Schalkhammer; Franz Rembert Aussenegg; Alfred Leitner; Harald Brunner; Gerhard Hawa; Christina Lobmaier; Fritz Pittner

doi:10.1117/12.275521

16 June 1997 Detection of fluorophore-labeled antibodies by surface-enhanced fluorescence on metal nanoislands

Thomas G. M. Schalkhammer, Franz Rembert Aussenegg, Alfred Leitner, Harald Brunner, Gerhard Hawa, Christina Lobmaier, Fritz Pittner

Author Affiliations +

Proceedings Volume 2976, Biomedical Sensing, Imaging, and Tracking Technologies II; (1997) https://doi.org/10.1117/12.275521
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

In fluorescence labelled immunosensing the discrimination of labels remaining in the bulk solution from labels bound to the analyte at the sensor surface is a basic optical problem. It is shown that application of surface enhanced fluorescence at a layer of noble metal nano-particles can increase the surface-to-background signal ratio. We explain the enhancement mechanism by an electrodynamic model and discuss the interaction between metal particle and fluorophore for the excitation and emission process. We show the principal guidelines for optimization of that processes. We find that the obtained discrimination power increases with decreasing intrinsic quantum efficiency of the fluorophore, suggesting the application of new classes of labels, namely low-quantum efficiency fluorophores. This theoretical finding is shown by a practical model experiment.

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Thomas G. M. Schalkhammer, Franz Rembert Aussenegg, Alfred Leitner, Harald Brunner, Gerhard Hawa, Christina Lobmaier, and Fritz Pittner "Detection of fluorophore-labeled antibodies by surface-enhanced fluorescence on metal nanoislands", Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); https://doi.org/10.1117/12.275521

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