Single-molecule adsorption at nanometer indentations

Mary Jean Wirth; Derrick J. Swinton; Melody D. Ludes; Leon J. Doneski; Cozette M. Cuppett; Hui Zhang

doi:10.1117/12.383336

21 April 2000 Single-molecule adsorption at nanometer indentations

Mary Jean Wirth, Derrick J. Swinton, Melody D. Ludes, Leon J. Doneski, Cozette M. Cuppett, Hui Zhang

Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383336
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The temporal behavior of bursts from single molecules reveals whether adsorbates are diffusing at chemical interfaces or are specifically adsorbed. The chemical interface of water/C₁₈-monolayer on silica is studied. Burst data for single molecules of DiI show that regions of the surface having more nanoindentations are associated with a greater number of specific adsorption events. Lysozyme is shown to adsorb irreversibly, and an oligonucleotide is shown to adsorb reversibly but more strongly than DiI to nanointendations at this interface. For each adsorbate, the specific adsorption at the nanoindentations is attributed to hydrogen bonding to exposed silanols.

Citation Download Citation

Mary Jean Wirth, Derrick J. Swinton, Melody D. Ludes, Leon J. Doneski, Cozette M. Cuppett, and Hui Zhang "Single-molecule adsorption at nanometer indentations", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); https://doi.org/10.1117/12.383336

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