CdSe quantum dot internalization by Bacillus subtilis and Escherichia coli

Jeremiah A. Kloepfer; Randall E. Mielke; Jay L. Nadeau

doi:10.1117/12.539152

14 June 2004 CdSe quantum dot internalization by Bacillus subtilis and Escherichia coli

Jeremiah A. Kloepfer, Randall E. Mielke, Jay L. Nadeau

Proceedings Volume 5361, Quantum Dots, Nanoparticles, and Nanoclusters; (2004) https://doi.org/10.1117/12.539152
Event: Integrated Optoelectronic Devices 2004, 2004, San Jose, CA, United States

Abstract

Biological labeling has been demonstrated with CdSe quantum dots in a variety of animal cells, but bacteria are harder to label because of their cell walls. We discuss the challenges of using minimally coated, bare CdSe quantum dots as luminescent internal labels for bacteria. These quantum dots were solubilized with mercaptoacetic acid and conjugated to adenine. Significant evidence for the internal staining of Bacillus subtilis (Gram positive) and Escherichia coli (Gram negative) using these structures is presented via steady-state emission, epifluorescence microscopy, transmission electron microscopy, and energy dispersive spectroscopy. In particular, the E. coli adenine auxotroph, and not the wild type, took up adenine coated quantum dots, and this only occurred in adenine deficient growth media. Labeling strength was enhanced by performing the incubation under room light. This process was examined with steady-state emission spectra and time-resolved luminescence profiles obtained from time-correlated-single-photon counting.

Citation Download Citation

Jeremiah A. Kloepfer, Randall E. Mielke, and Jay L. Nadeau "CdSe quantum dot internalization by Bacillus subtilis and Escherichia coli", Proc. SPIE 5361, Quantum Dots, Nanoparticles, and Nanoclusters, (14 June 2004); https://doi.org/10.1117/12.539152

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