Monitoring cell profile in tissue engineered constructs by OCT

Ying Yang; Pierre O. Bagnaninchi; Mairead A. Wood; Alicia J. El Haj; Elvire Guyot; Arnaud Dubois; Ruikang K. Wang

doi:10.1117/12.592752

15 April 2005 Monitoring cell profile in tissue engineered constructs by OCT

Ying Yang, Pierre O. Bagnaninchi, Mairead A. Wood, Alicia J. El Haj, Elvire Guyot, Arnaud Dubois, Ruikang K. Wang

Proceedings Volume 5695, Optical Interactions with Tissue and Cells XVI; (2005) https://doi.org/10.1117/12.592752
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Despite significant progress in tissue engineering over the last decade, the development of real-time, non-destructive tools for monitoring the development of engineered tissues remains a great challenge. To date, the evaluation of cell proliferation and extracellular matrix production in response to various culture conditions depends upon traditional DNA, RNA and protein analysis which requires extraction of cell components from constructs resulting in loss of tissue morphology and integrity. In this study, we report how optical coherence tomography (OCT) can be exploited to monitor cell profiles in real-time and in a non-destructive manner. Scaffolds made from poly(lactic acid) (PLLA) with various porosities were scanned by OCT. A local porosity analysis method has been developed to quantify the porosity change. The hypothesis is whether the local porosity analysis can correlate with the tissue growth within the scaffold following seeding of the cells within it. Bone cells have been grown in the PLLA scaffolds under different culture conditions. The OCT images of these scaffolds have been collected. It has been found that the porosity of the cultured scaffold-cell constructs reduced under different culture conditions compared to blank scaffolds. A decrease in light penetration depth in OCT images has also been observed. There existed a good relationship between the local porosity and tissue growth. It has been demonstrated that the mean local porosity based on OCT images can become a unique method to correlate and monitor tissue growth.

Citation Download Citation

Ying Yang, Pierre O. Bagnaninchi, Mairead A. Wood, Alicia J. El Haj, Elvire Guyot, Arnaud Dubois, and Ruikang K. Wang "Monitoring cell profile in tissue engineered constructs by OCT", Proc. SPIE 5695, Optical Interactions with Tissue and Cells XVI, (15 April 2005); https://doi.org/10.1117/12.592752

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