Are luminescent quantum dots efficient energy acceptors?

Aaron R. Clapp; Igor L. Medintz; Brent R. Fisher; Hedi Mattoussi

doi:10.1117/12.590989

28 April 2005 Are luminescent quantum dots efficient energy acceptors?

Aaron R. Clapp, Igor L. Medintz, Brent R. Fisher, Hedi Mattoussi

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.590989
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Steady state and time-resolved fluorescence measurements were used to investigate the ability of luminescent quantum dots (QDs) to function as efficient energy acceptors in fluorescence resonance energy transfer (FRET) binding assays with organic dye donors. Fluorescent dyes, AlexaFluor 488 or Cy3, were used with various QD acceptors in QD-dye-labeled-protein conjugates. Data derived from both sets of experiments showed no apparent FRET from dye to QD. The collected data were discussed within the framework of a competition between a fast radiative decay rate of the donor excitation and a slower FRET decay rate. This is due to the long exciton lifetime of the acceptor compared to that of the dye, combined with substantial QD direct excitation.

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Aaron R. Clapp, Igor L. Medintz, Brent R. Fisher, and Hedi Mattoussi "Are luminescent quantum dots efficient energy acceptors?", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.590989

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KEYWORDS

Fluorescence resonance energy transfer

Proteins

Luminescence

Excitons

Absorption

Time resolved spectroscopy

Quantum dots

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