Optical tweezers and multiphoton microscopies integrated photonic tool for mechanical and biochemical cell processes studies

A. A. de Thomaz; W. M. Faustino; A. Fontes; H. P. Fernandes; M. d. L. Barjas-Castro; K. Metze; S. Giorgio; L. C. Barbosa; C. L. Cesar

doi:10.1117/12.734495

5 September 2007 Optical tweezers and multiphoton microscopies integrated photonic tool for mechanical and biochemical cell processes studies

A. A. de Thomaz, W. M. Faustino, A. Fontes, H. P. Fernandes, M. d. L. Barjas-Castro, K. Metze, S. Giorgio, L. C. Barbosa, C. L. Cesar

Author Affiliations +

Proceedings Volume 6644, Optical Trapping and Optical Micromanipulation IV; 66440H (2007) https://doi.org/10.1117/12.734495
Event: NanoScience + Engineering, 2007, San Diego, California, United States

Abstract

The research in biomedical photonics is clearly evolving in the direction of the understanding of biological processes at the cell level. The spatial resolution to accomplish this task practically requires photonics tools. However, an integration of different photonic tools and a multimodal and functional approach will be necessary to access the mechanical and biochemical cell processes. This way we can observe mechanicaly triggered biochemical events or biochemicaly triggered mechanical events, or even observe simultaneously mechanical and biochemical events triggered by other means, e.g. electricaly. One great advantage of the photonic tools is its easiness for integration. Therefore, we developed such integrated tool by incorporating single and double Optical Tweezers with Confocal Single and Multiphoton Microscopies. This system can perform 2-photon excited fluorescence and Second Harmonic Generation microscopies together with optical manipulations. It also can acquire Fluorescence and SHG spectra of specific spots. Force, elasticity and viscosity measurements of stretched membranes can be followed by real time confocal microscopies. Also opticaly trapped living protozoas, such as leishmania amazonensis. Integration with CARS microscopy is under way. We will show several examples of the use of such integrated instrument and its potential to observe mechanical and biochemical processes at cell level.

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A. A. de Thomaz, W. M. Faustino, A. Fontes, H. P. Fernandes, M. d. L. Barjas-Castro, K. Metze, S. Giorgio, L. C. Barbosa, and C. L. Cesar "Optical tweezers and multiphoton microscopies integrated photonic tool for mechanical and biochemical cell processes studies", Proc. SPIE 6644, Optical Trapping and Optical Micromanipulation IV, 66440H (5 September 2007); https://doi.org/10.1117/12.734495

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