Optical characterization of porous silicon microcavities for glucose oxidase biosensing

G. Palestino; V. Agarwal; D. B. Garcia; R. Legros; E. Pérez; C. Gergely

doi:10.1117/12.781045

8 May 2008 Optical characterization of porous silicon microcavities for glucose oxidase biosensing

G. Palestino, V. Agarwal, D. B. Garcia, R. Legros, E. Pérez, C. Gergely

Proceedings Volume 6991, Biophotonics: Photonic Solutions for Better Health Care; 69911Y (2008) https://doi.org/10.1117/12.781045
Event: SPIE Photonics Europe, 2008, Strasbourg, France

Abstract

PSi microcavity (PSiMc) is characterized by a narrow resonance peak in the optical spectrum that is very sensitive to small changes in the refractive index. We report that the resonant optical cavities of PSi structures can be used to enhance the detection of labeled fluorescent biomolecules. Various PSi configurations were tested in order to compare the optical response of the PSi devices to the capture of organic molecules. Morphological and topographical analyses were performed on PSiMc using Atomic Force (AFM) and Scanning Electron (SEM) microscopies. The heterogeneity in pores lengths resulting from etching process assures a better penetration of larger molecules into the pores and sensor sensitivity depends on the pore size. Molecular detection is monitored by the successive red shifts in the reflectance spectra after the stabilization of PSiMc with 3-aminopropyltriethoxysilane (APTES). The glucose oxidase was cross linked into the PSiMc structures following a silane-glutaraldehyde (GTA) chemistry.

Citation Download Citation

G. Palestino, V. Agarwal, D. B. Garcia, R. Legros, E. Pérez, and C. Gergely "Optical characterization of porous silicon microcavities for glucose oxidase biosensing", Proc. SPIE 6991, Biophotonics: Photonic Solutions for Better Health Care, 69911Y (8 May 2008); https://doi.org/10.1117/12.781045

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available