Label-free biosensing using a photonic crystal structure in a total-internal-reflection geometry

Yunbo Guo; Jing Yong Ye; Charles Divin; Thommey P. Thomas; Andrzej Myc; Tommaso F. Bersano-Begey; James R. Baker Jr.; Theodore B. Norris

doi:10.1117/12.808369

16 February 2009 Label-free biosensing using a photonic crystal structure in a total-internal-reflection geometry

Yunbo Guo, Jing Yong Ye, Charles Divin, Thommey P. Thomas, Andrzej Myc, Tommaso F. Bersano-Begey, James R. Baker Jr., Theodore B. Norris

Author Affiliations +

Proceedings Volume 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI; 71880B (2009) https://doi.org/10.1117/12.808369
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

A novel optical biosensor using a one-dimensional photonic crystal structure in a total-internal-reflection geometry (PCTIR) is presented and investigated for label-free biosensing applications. This simple configuration forms a micro Fabry- Perot resonator in the top layer which provides a narrow optical resonance to enable label-free, highly sensitive measurements for the presence of analytes on the sensing surface or the refractive index change of the surrounding medium in the enhanced evanescent field; and at the same time it employs an open sensing surface for real-time biomolecular binding detection. The high sensitivity of the sensor was experimentally demonstrated by bulk solvent refractive index changes, ultrathin molecular films adsorbed on the sensing surface, and real-time analytes binding, measuring both the spectral shift of the photonic crystal resonance and the change of the intensity ratio in a differential reflectance measurement. Detection limits of 7×10^-8 RIU for bulk solvent refractive index, 6×10^-5 nm for molecular layer thickness and 24 fg/mm² for mass density were obtained, which represent a significant improvement relative to state-ofthe- art surface-plasmon-resonance (SPR)-based systems. The PC-TIR sensor is thus seen to be a promising technology platform for high sensitivity and accurate biomolecular detection.

Citation Download Citation

Yunbo Guo, Jing Yong Ye, Charles Divin, Thommey P. Thomas, Andrzej Myc, Tommaso F. Bersano-Begey, James R. Baker Jr., and Theodore B. Norris "Label-free biosensing using a photonic crystal structure in a total-internal-reflection geometry", Proc. SPIE 7188, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications VI, 71880B (16 February 2009); https://doi.org/10.1117/12.808369

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