CdTe/CdS-MPA quantum dots as fluorescent probes to label yeast cells: synthesis, characterization and conjugation with Concanavalin A

Ilka T. Kato; Camila C. Santos; Endi Benetti; Denise P. L. A. Tenório; Paulo E. Cabral Filho; Caetano P. Sabino; Adriana Fontes; Beate S. Santos; Renato A. Prates; Martha S. Ribeiro

doi:10.1117/12.909060

2 February 2012 CdTe/CdS-MPA quantum dots as fluorescent probes to label yeast cells: synthesis, characterization and conjugation with Concanavalin A

Ilka T. Kato, Camila C. Santos, Endi Benetti, Denise P. L. A. Tenório, Paulo E. Cabral Filho, Caetano P. Sabino, Adriana Fontes, Beate S. Santos, Renato A. Prates, Martha S. Ribeiro

Author Affiliations +

Proceedings Volume 8232, Colloidal Nanocrystals for Biomedical Applications VII; 82320D (2012) https://doi.org/10.1117/12.909060
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Candida albicans is the most frequent human opportunistic pathogenic fungus and one of the most important causes of nosocomial infections. In fact, diagnosis of invasive candidiasis presents unique problems. The aim of this work was to evaluate, by fluorescence image analysis, cellular labeling of C. albicans with CdTe/CdS quantum dots conjugated or not to concanavalin A (ConA). Yeast cells were incubated with CdTe/CdS quantum dots (QD) stabilized with mercaptopropionic acid (MPA) (emission peak at 530 nm) for 1 hour. In the overall study we observed no morphological alterations. The fluorescence microscopic analysis of the yeast cells showed that the non-functionalized QDs do not label C. albicans cells, while for the QD conjugated to ConA the cells showed a fluorescence profile indicating that the membrane was preferentially marked. This profile was expected since Concanavalin A is a protein that binds specifically to terminal carbohydrate residues at the membrane cell surface. The results suggest that the QD-labeled Candida cells represent a promising tool to open new possibilities for a precise evaluation of fungal infections in pathological conditions.

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Ilka T. Kato, Camila C. Santos, Endi Benetti, Denise P. L. A. Tenório, Paulo E. Cabral Filho, Caetano P. Sabino, Adriana Fontes, Beate S. Santos, Renato A. Prates, and Martha S. Ribeiro "CdTe/CdS-MPA quantum dots as fluorescent probes to label yeast cells: synthesis, characterization and conjugation with Concanavalin A", Proc. SPIE 8232, Colloidal Nanocrystals for Biomedical Applications VII, 82320D (2 February 2012); https://doi.org/10.1117/12.909060

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