Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli

R. B. Queirós; C. Gouveia; J. R. A. Fernandes; P. A. S. Jorge

doi:10.1117/12.2025813

20 May 2013 Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli

R. B. Queirós, C. Gouveia, J. R. A. Fernandes, P. A. S. Jorge

Author Affiliations +

Proceedings Volume 8794, Fifth European Workshop on Optical Fibre Sensors; 879414 (2013) https://doi.org/10.1117/12.2025813
Event: Fifth European Workshop on Optical Fibre Sensors, 2013, Krakow, Poland

Abstract

An evanescent wave fiber optic sensor for detection of E. coli outer membranes proteins (EcOMPs) is presented. The sensing probe is achieved by the functionalization of a Long Period Grating (LPG) inscribed in a single mode fiber (SMF28) with poly-L-lysine (PLL) resulting in a label-free configuration capable of specific recognition of EcOMPs in waters due to the resonance wavelength shift variation owing to refractive index changes of the medium (≈100 nm/RIU). The sensing head was characterized and tested against EcOMP and applied to spiked environmental water samples. The sensor displayed linear responses in the range of 1×10^-10M to 1×10^-8 M EcOMP and is regenerated (under low pH conditions) and the deviation of the subsequent detection was less than 0.1 %.

Citation Download Citation

R. B. Queirós, C. Gouveia, J. R. A. Fernandes, and P. A. S. Jorge "Evanescent wave DNA-aptamer biosensor based on long period gratings for the specific recognition of E. coli", Proc. SPIE 8794, Fifth European Workshop on Optical Fibre Sensors, 879414 (20 May 2013); https://doi.org/10.1117/12.2025813

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