We developed an agarose based mounting system that holds the embryos in defined positions along removable strips. Homeostasis and temperature control is ensured by channels circulating buffer and heated water. This allows to image up to 120 larvae simultaneously for more than two days. Its flexibility and the low-volume, high larvae ratio will allow screening of small compound libraries. Taken together, we offer a low cost, highly adaptable solution for long term in-vivo imaging. |
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CITATIONS
Cited by 1 scholarly publication.
Temperature metrology
3D printing
Microscopes
Spinal cord
Computer aided design
Tissues
Imaging systems