High-contrast visualization of human skin cancers with combined reflectance confocal and moxifloxacin based two-photon microscopy

Won Yeong Park; Bumju Kim; Jihyun Chun; Seung-Mo Hong; Byung Ho Oh; Ki Hean Kim

doi:10.1117/12.2611039

7 March 2022 High-contrast visualization of human skin cancers with combined reflectance confocal and moxifloxacin based two-photon microscopy

Won Yeong Park, Bumju Kim, Jihyun Chun, Seung-Mo Hong, Byung Ho Oh, Ki Hean Kim

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Proceedings Volume PC11934, Photonics in Dermatology and Plastic Surgery 2022; PC119340E (2022) https://doi.org/10.1117/12.2611039
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Mohs Micrographic Surgery (MMS) needs optical biopsy methods for tumor margin determination. Although confocal microscopy CM) has been used, CM has poor contrast to detect cancer cells with reflection contrast. We developed combined reflectance confocal (RC) & Moxifloxacin based two-photon (MB-TP) microscopy for high contrast. Here, combined microscopy was tested in various skin cancer tissues. Combined microscopy visualized both cell & extracellular matrix. Basal cell carcinoma nests were detected and distinguished from glands. Squamous cell carcinoma was detected with some features. This study showed combined microscopy has potentials for guiding MMS.

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Won Yeong Park, Bumju Kim, Jihyun Chun, Seung-Mo Hong, Byung Ho Oh, and Ki Hean Kim "High-contrast visualization of human skin cancers with combined reflectance confocal and moxifloxacin based two-photon microscopy", Proc. SPIE PC11934, Photonics in Dermatology and Plastic Surgery 2022, PC119340E (7 March 2022); https://doi.org/10.1117/12.2611039

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KEYWORDS

Skin cancer

Visualization

Tissues

Confocal microscopy

Reflectivity

Two photon excitation microscopy

Skin

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