Methylene blue fluorescence polarization assessment of breast cancer tumor grades and molecular subtypes (Conference Presentation)

Peter Jermain; Dina Kandil; Alona Muzikansky; Ashraf Khan; Anna N. Yaroslavsky

doi:10.1117/12.2651796

15 March 2023 Methylene blue fluorescence polarization assessment of breast cancer tumor grades and molecular subtypes (Conference Presentation)

Peter Jermain, Dina Kandil, Alona Muzikansky, Ashraf Khan, Anna N. Yaroslavsky

Author Affiliations +

Proceedings Volume PC12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023; PC1238205 (2023) https://doi.org/10.1117/12.2651796
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Examination of breast fine-needle aspiration (FNA) cytology specimens requires subjective assessment of cell morphology. Consequently, low diagnostic accuracy and high error rates (~40%) have been reported. We investigated methylene blue (MB) fluorescence polarization (Fpol) values depending on tumor grades/molecular subtypes. Pathologically diverse FNA specimens were collected from surgically excised breast tissues, stained in MB, and imaged. MB Fpol images provided a quantitative measurement for each cell. Optical imaging results were compared to clinical assessments. Statistical analysis revealed correlation between MB Fpol values and tumor grade/molecular subtype. The findings indicate that this method could provide accurate differentiation of breast cytology specimens.

Conference Presentation

Citation Download Citation

Peter Jermain, Dina Kandil, Alona Muzikansky, Ashraf Khan, and Anna N. Yaroslavsky "Methylene blue fluorescence polarization assessment of breast cancer tumor grades and molecular subtypes (Conference Presentation)", Proc. SPIE PC12382, Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2023, PC1238205 (15 March 2023); https://doi.org/10.1117/12.2651796

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available