Fast-acquisition Raman methodologies such as stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy, which generate image contrast using the Raman active vibrational frequency of a given chemical, provide information on the biochemical composition of tissues. This has enabled detailed imaging of the interaction between tumor cells and the surrounding tumor microenvironment (e.g. tumor vasculature, stromal cells) without the need for additional labelling. In addition, we have developed novel, highly Raman active spectroscopically bioorthogonal labels, for the sensitive and specific intracellular visualisation of small-molecules and cells, allowing monitoring of drug uptake and tracking of individual cell populations.
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