Optical metabolic imaging of neutrophil activation and effector function

Rupsa Datta

doi:10.1117/12.3001813

13 March 2024 Optical metabolic imaging of neutrophil activation and effector function

Rupsa Datta

Author Affiliations +

Proceedings Volume PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV; PC128470Q (2024) https://doi.org/10.1117/12.3001813
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

This study employs the optical redox ratio and fluorescence lifetime imaging microscopy of intrinsic metabolic coenzymes NAD(P)H and FAD to assess the metabolic state of human neutrophils in vitro, under a variety of activation conditions and targeted metabolic pathway inhibition at the single-cell level. By measuring effector functions such as oxidative burst, neutrophil activation could be confirmed. In addition, identical metabolic changes were detected in zebrafish larvae neutrophils following activation, confirming the validity of these findings in vivo.

Conference Presentation

Citation Download Citation

Rupsa Datta "Optical metabolic imaging of neutrophil activation and effector function", Proc. SPIE PC12847, Multiphoton Microscopy in the Biomedical Sciences XXIV, PC128470Q (13 March 2024); https://doi.org/10.1117/12.3001813

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