In situ monitoring of collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held, second-harmonic-generation microscope

K. Atsuta; Y. Ogura; E. Hase; T. Minamikawa; T. Yasui

doi:10.1117/12.2251321

21 February 2017 In situ monitoring of collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held, second-harmonic-generation microscope

K. Atsuta, Y. Ogura, E. Hase, T. Minamikawa, T. Yasui

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100692B (2017) https://doi.org/10.1117/12.2251321
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Second-harmonic-generation (SHG) microscopy is a powerful tool for in situ monitoring of collagen fibers in human skin. However, its practical use is still limited in the dermatological field due to the bulky and complicated setup. In this paper, we constructed a photonic-crystal-fiber-coupled, hand-held SHG microscope for in situ monitoring of collagen fibers in human skin. Fiber delivery of femtosecond pulse light was achieved by a large-mode-area photonic-crystal-fiber whereas the compact SHG microscopy setup was enclosed into a lens tube system. The combination of PCF with the lens tube system largely enhances the flexibility of measurement sites in the human skin.

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K. Atsuta, Y. Ogura, E. Hase, T. Minamikawa, and T. Yasui "In situ monitoring of collagen fibers in human skin using a photonic-crystal-fiber-coupled, hand-held, second-harmonic-generation microscope", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692B (21 February 2017); https://doi.org/10.1117/12.2251321

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