Image Processing Of Transmission Electron Micrographs Using The Semper V Interactive Display System

Mohamed Shawki Elghazali

doi:10.1117/12.950482

14 April 1989 Image Processing Of Transmission Electron Micrographs Using The Semper V Interactive Display System

Mohamed Shawki Elghazali

Proceedings Volume 1030, Biostereometrics '88; (1989) https://doi.org/10.1117/12.950482
Event: Biostereometrics '88: Spatial and Spatiotemporal Analysis of Biological Form and Function, 1988, Basel, Switzerland

Abstract

There is a growing demand among electron microscopic users to extract spatial information from photographed micro objects. Methods utilizing the well established photogrammetric theories either using analog or analytical approaches have been successful with electron micrographs. However, these methods are rather time consuming particularly for these applications that require mapping of large number of micrographs. In this paper an interactive display system (Semper V) composed of a camera an IBM/PC microcomputer together with software developed by Image Techniques of Cambridge Ltd is used as an image processing system performing image enhancement and filtering, masking, histograms, as well as gray scaled contouring. Transmission electron microscopes produce highly magnified images that resemble X-ray photography revealing different gray levels proportional to the thickness of the object, thus making them very suited to the prorosed system: Comparison of mapping a microscopic object using Kern DSR 11 analytical plotter and the proposed interactive display system is presented. Results proved to be comparable in accuracy while the time saving is dramatic making this system very attractive to several users.

Citation Download Citation

Mohamed Shawki Elghazali "Image Processing Of Transmission Electron Micrographs Using The Semper V Interactive Display System", Proc. SPIE 1030, Biostereometrics '88, (14 April 1989); https://doi.org/10.1117/12.950482

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