Insight into microenvironment of tumor on the microscopic level with a focus on cancer-associated fibroblasts

Irina  N. Druzhkova; Maria M. Lukina; Varvara Dudenkova; Lubov E. Shimolina; Elena V. Zagaynova M.D.; Marina V. Shirmanova

doi:10.1117/12.2307096

17 May 2018 Insight into microenvironment of tumor on the microscopic level with a focus on cancer-associated fibroblasts

Irina N. Druzhkova, Maria M. Lukina, Varvara Dudenkova, Lubov E. Shimolina, Elena V. Zagaynova M.D., Marina V. Shirmanova

Author Affiliations +

Proceedings Volume 10685, Biophotonics: Photonic Solutions for Better Health Care VI; 106852R (2018) https://doi.org/10.1117/12.2307096
Event: SPIE Photonics Europe, 2018, Strasbourg, France

Abstract

Cancer-associated fibroblasts (CAFs) are one of the key determinants in the malignant progression of cancer. The subject of this research was metabolic reorganization of CAFs and their participation in collagen cross-linking process. The metabolic differences between normal fibroblasts and CAFs were elucidated using two-photon fluorescence lifetime imaging microscopy (FLIM). Collagen structure in 3D model was assessed using second harmonic generation (SHG) microscopy. We show increased metabolic activity of fibroblasts derived from patient’s colon tumor with a shift to more oxidative metabolism compare to dermal fibroblasts. The results of the study of collagen suggest that CAFs may contribute to the tumor progression through the facilitation of collagen alignment. In general, our findings support the idea of the strong association between cancer cells and fibroblasts and extensive involvement of CAFs in modulation of tumor microenvironment.

Citation Download Citation

Irina N. Druzhkova, Maria M. Lukina, Varvara Dudenkova, Lubov E. Shimolina, Elena V. Zagaynova M.D., and Marina V. Shirmanova "Insight into microenvironment of tumor on the microscopic level with a focus on cancer-associated fibroblasts", Proc. SPIE 10685, Biophotonics: Photonic Solutions for Better Health Care VI, 106852R (17 May 2018); https://doi.org/10.1117/12.2307096

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available