Enhanced collection efficiency of vesicles in a suspension by optical pressure using gold nanoparticles

Takashi Kaneta; Mai Kuboi; Nobuyuki Takeyasu

doi:10.1117/12.2317724

24 April 2018 Enhanced collection efficiency of vesicles in a suspension by optical pressure using gold nanoparticles

Takashi Kaneta, Mai Kuboi, Nobuyuki Takeyasu

Proceedings Volume 10712, Optical Manipulation Conference; 1071205 (2018) https://doi.org/10.1117/12.2317724
Event: SPIE Structured Light, 2018, Yokohama, Japan

Abstract

We developed a method to collect micro- and nano-vesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on the glass substrate which sandwiches a suspension containing micro- or nano-sized vesicles prepared by a phospholipid. The optical pressure generated at the interface of the medium and the vesicles accelerated the vesicles to form aggregates on the glass surface. Two types of glass substrates, hydrophilic and aminated ones, showed no difference in the adsorption property of the vesicles. Time to be required for collecting a certain amount of the vesicles was inversely proportional to the concentration of the vesicles. To enhance the collection efficiency, gold nanoparticles were added to the suspension of the vesicles. We found that gold nanoparticles reduced the collection time as short as 1/10-times.

Citation Download Citation

Takashi Kaneta, Mai Kuboi, and Nobuyuki Takeyasu "Enhanced collection efficiency of vesicles in a suspension by optical pressure using gold nanoparticles", Proc. SPIE 10712, Optical Manipulation Conference, 1071205 (24 April 2018); https://doi.org/10.1117/12.2317724

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