Interrogation of glioma metabolism on macroscale by FLIM

Marina V. Shirmanova; Maria Lukina; Elena B. Kisileva; Vera V Fedoseeva; Varvara V. Dudenkova; Elena V. Zagaynova; Wolfgang Becker; Vladislav I. Shcheslavskiy

doi:10.1117/12.2511475

22 February 2019 Interrogation of glioma metabolism on macroscale by FLIM

Marina V. Shirmanova, Maria Lukina, Elena B. Kisileva, Vera V Fedoseeva, Varvara V. Dudenkova, Elena V. Zagaynova, Wolfgang Becker, Vladislav I. Shcheslavskiy

Author Affiliations +

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 1088209 (2019) https://doi.org/10.1117/12.2511475
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

This paper presents ex-vivo investigation of the brain tumors, namely glioblastoma and anaplastic astrocytoma, by macroscopic fluorescence lifetime imaging (FLIM) of endogenous metabolic cofactor nicotine amide dinucleotide (phosphate), NAD(P)H. The results of the study on the rat models indicate that the metabolism in brain tumors differs between tumor types and differs from normal brain tissue. It was also found that the brain tumors have specific optical metabolic signatures that differ them from most solid tumors, underling the complexity of glioma metabolism. The approach used in the experiments shows prospects to determine the surgical margins of gliomas and to investigate metabolic heterogeneity of the tumors on a macroscale. However, one has to be careful in the interpretation of the data obtained by FLIM.

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Marina V. Shirmanova, Maria Lukina, Elena B. Kisileva, Vera V Fedoseeva, Varvara V. Dudenkova, Elena V. Zagaynova, Wolfgang Becker, and Vladislav I. Shcheslavskiy "Interrogation of glioma metabolism on macroscale by FLIM", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 1088209 (22 February 2019); https://doi.org/10.1117/12.2511475

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