Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics

Priyank Shah; Brad Littleton; Thomas Kavanagh; Yu Nie; Stephen Sturzenbaum; David Richards

doi:10.1117/12.2510052

22 February 2019 Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics

Priyank Shah, Brad Littleton, Thomas Kavanagh, Yu Nie, Stephen Sturzenbaum, David Richards

Proceedings Volume 10882, Multiphoton Microscopy in the Biomedical Sciences XIX; 108822C (2019) https://doi.org/10.1117/12.2510052
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

Broadband coherent anti-Stokes Raman scattering (B-CARS) in a hybrid 2-color/3-color excitation regime has been shown to be a photon-efficient method of generating CARS across the entire vibrational region of interest (fingerprint and C-H stretch). We extend here our spectral interferometric polarized CARS (SIP-CARS) approach to a hybrid 2-color/3-color excitation regime, demonstrate that the power/polarization dependence for both 2- color and 3-color excitation agrees with theory, explore the application of SIP-CARS for the special case of anisotropic, birefringent materials (using collagen) and present in vivo lipid imaging of different mutations of the nematode Caenorhabditis elegans with modified lipid distributions. The extension of SIP-CARS to a hybrid excitation scheme makes possible a fast, all-optical and quantitative method for Raman imaging.

Citation Download Citation

Priyank Shah, Brad Littleton, Thomas Kavanagh, Yu Nie, Stephen Sturzenbaum, and David Richards "Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics", Proc. SPIE 10882, Multiphoton Microscopy in the Biomedical Sciences XIX, 108822C (22 February 2019); https://doi.org/10.1117/12.2510052

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