Imaging single nano-emitters and single cells by electrochemiluminescence microscopy (Conference Presentation)

Junjie Zhu; Cheng Ma

doi:10.1117/12.2508621

13 March 2019 Imaging single nano-emitters and single cells by electrochemiluminescence microscopy (Conference Presentation)

Junjie Zhu, Cheng Ma

Author Affiliations +

Proceedings Volume 10892, Colloidal Nanoparticles for Biomedical Applications XIV; 108920X (2019) https://doi.org/10.1117/12.2508621
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

The quest for new techniques to measure single nanoparticle is a great impetus to research efforts to understand individual behaviors. Herein, we develop an electrochemiluminescence (ECL) microscopy for visualization of stochastic collision electrochemistry of single nano-emitters without the interference of current and optical background. This design uses a water-immersion objective to capture the ECL emission of nanoparticles near the specular electrode surface for enhancing light collection efficiency. The approach enables us to trace the collision trajectory of multiple nanoparticles and spatially distinguish simultaneous collisions. This imaging technique displays great potential for applications in single-particle electrochemical and analytical research. In addition to imaging single nano-emitters, we perform single cell imaging by the homemade ECL microscope. Since cells are immobilized on the electrode surface, the steric hindrance and the insulation from the cells make it difficult to obtain a luminous cell ECL image. To solve this problem, direct ECL imaging of a single cell was investigated and achieved on chitosan and nano-TiO2 modified fluoride-doped tin oxide conductive glass (FTO/TiO2/CS). The permeable chitosan film is not only favorable for cell immobilization but also increases the space between the bottom of cells and the electrode; thus, more ECL reagent can exist below the cells compared with the cells on a bare electrode, which guarantees the high sensitivity of quantitative analysis. The light intensity is correlated with the H2O2 concentration on FTO/TiO2/CS, which can be applied to analyze the H2O2 released from cells at the single-cell level.

Conference Presentation

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Junjie Zhu and Cheng Ma "Imaging single nano-emitters and single cells by electrochemiluminescence microscopy (Conference Presentation)", Proc. SPIE 10892, Colloidal Nanoparticles for Biomedical Applications XIV, 108920X (13 March 2019); https://doi.org/10.1117/12.2508621

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KEYWORDS

Microscopy

Electrodes

Nanoparticles

Analytical research

Glasses

Microscopes

Oxides

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