Gas-chromatography-tandem mass spectrometry (GC-MS) method for the determination of glycerol in human urine samples

Manuela Dobrescu; Mirela Zorio; Mihai Ionica

doi:10.1117/12.2324708

31 December 2018 Gas-chromatography-tandem mass spectrometry (GC-MS) method for the determination of glycerol in human urine samples

Manuela Dobrescu, Mirela Zorio, Mihai Ionica

Author Affiliations +

Proceedings Volume 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX; 109773G (2018) https://doi.org/10.1117/12.2324708
Event: Advanced Topics in Optoelectronics, Microelectronics and Nanotechnologies IX, 2018, Constanta, Romania

Abstract

Glycerol is the key constituent to triglycerides and is an important compound participating in the lipid metabolism. It is an endogenous compound found in small amounts in human blood and urine. In high doses it can cause migraine, nausea, thirst¹. The present degree has purpose the developing and validating a Gas-Chromatography- Tandem Mass Spectrometry (GC-MS) method accurate, rapid and easy to identify and quantify the glycerol analyte in urine samples. The applied analytical technique is based on derivation of the urine sample evaporated with MSTFA/NH₄I/ethantiol derivatizing reagent. By this reaction, the hydroxyl functions of glycerol are converted to silyl derivatives, compounds having boiling points lower than the original compounds, this facilitating their GC-MS assay. For estimating the glycerol content, the method are monitored the mass fragments characteristic of the compound of interest – m/z 205, 218 and 293 and an ion corresponding to the deurated analog-m/z 208².

Citation Download Citation

Manuela Dobrescu, Mirela Zorio, and Mihai Ionica "Gas-chromatography-tandem mass spectrometry (GC-MS) method for the determination of glycerol in human urine samples", Proc. SPIE 10977, Advanced Topics in Optoelectronics, Microelectronics, and Nanotechnologies IX, 109773G (31 December 2018); https://doi.org/10.1117/12.2324708

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