Marker-free automatic quantification of red blood cell fluctuations with different storage periods by holographic imaging

Inkyu Moon; Keyvan Jaferzadeh

doi:10.1117/12.2516153

14 May 2019 Marker-free automatic quantification of red blood cell fluctuations with different storage periods by holographic imaging

Inkyu Moon, Keyvan Jaferzadeh

Author Affiliations +

Proceedings Volume 10997, Three-Dimensional Imaging, Visualization, and Display 2019; 109970J (2019) https://doi.org/10.1117/12.2516153
Event: SPIE Defense + Commercial Sensing, 2019, Baltimore, MD, United States

Abstract

This paper overviews the methods to quantitatively measure the cell membrane fluctuation (CMF) rate of red blood cells (RBCs) with different storage periods with millisecond temporal sensitivity at the single-cell level by using marker-free holographic imaging techniques. We quantitatively measured fluctuations in the discocyte shape of RBCs membrane, ring and dimple in the case of storage lesion with time-lapse phase images of RBCs. Our experimental results demonstrate that normal RBCs with a discocyte shape become stiffer with storage period and there is a significant negative correlation between CMFs and the sphericity coefficient, which describes the RBCs morphology. Correlation between CMF and projected surface area is also performed.

Conference Presentation

Citation Download Citation

Inkyu Moon and Keyvan Jaferzadeh "Marker-free automatic quantification of red blood cell fluctuations with different storage periods by holographic imaging", Proc. SPIE 10997, Three-Dimensional Imaging, Visualization, and Display 2019, 109970J (14 May 2019); https://doi.org/10.1117/12.2516153

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available