Design and evaluation of two imaging systems for very wide field fluorescence microscopy

Isaure de Kernier; Nelly Rongeat; Sophie Morales; Serge Monneret; Pierre Blandin

doi:10.1117/12.2526938

22 July 2019 Design and evaluation of two imaging systems for very wide field fluorescence microscopy

Isaure de Kernier, Nelly Rongeat, Sophie Morales, Serge Monneret, Pierre Blandin

Author Affiliations +

Proceedings Volume 11075, Novel Biophotonics Techniques and Applications V; 110750A (2019) https://doi.org/10.1117/12.2526938
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Very wide field of view imaging can be used in biology to infer statistical information on cell populations from a singleshot acquisition. In particular, for applications in hematology, fluorescence wide-field of view imaging could be an alternative to standard fluorescence flow cytometry methods; it can be useful to achieve standard blood tests, such a leukocyte count or leukocyte differential count. We will introduce two 30mm² wide-field fluorescence imaging set-ups and compare their performances. Both systems achieve 1x magnification. The first one is based on a macro-photography lens. Although such a system optimizes the resolution and field of view, it suffers from its bulkiness. With the second system, we seek miniaturization while loosening the requirements on image quality. It is based on a lens-less approach with a fiber plate optical relay. The potential of the two systems for hematology analyzes will be illustrated with the imaging of labelled white blood cells.

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Isaure de Kernier, Nelly Rongeat, Sophie Morales, Serge Monneret, and Pierre Blandin "Design and evaluation of two imaging systems for very wide field fluorescence microscopy", Proc. SPIE 11075, Novel Biophotonics Techniques and Applications V, 110750A (22 July 2019); https://doi.org/10.1117/12.2526938

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