Terbium ion as RNA tag for slide-free histology with deep-ultraviolet excitation fluorescence (Conference Presentation)

Yasuaki Kumamoto

doi:10.1117/12.2544487

9 March 2020 Terbium ion as RNA tag for slide-free histology with deep-ultraviolet excitation fluorescence (Conference Presentation)

Yasuaki Kumamoto

Author Affiliations +

Proceedings Volume 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis; 1123411 (2020) https://doi.org/10.1117/12.2544487
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Terbium ions, Tb3+, fluoresced in a cell at deep-ultraviolet excitation. Intensity of the fluorescence was dropped by RNA decomposition. It was also found that fluorescence intensity of Tb3+ conjugated with DNA increased with strand dissociation. These results indicated specificity of Tb3+ to single-stranded nucleic acid, mostly RNA in cell and tissue. We employed Tb3+ and Hoechst, DNA-specific dyes, for deep-ultraviolet excitation fluorescence imaging. With this imaging, nucleolus, nucleoplasm, and cytoplasm were discriminated. Thanks to the small penetration depth of deep-ultraviolet light into tissue, this imaging modality was useful for slide-free histology and rapid cancer detection in a human lymph node.

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Yasuaki Kumamoto "Terbium ion as RNA tag for slide-free histology with deep-ultraviolet excitation fluorescence (Conference Presentation)", Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1123411 (9 March 2020); https://doi.org/10.1117/12.2544487

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KEYWORDS

Luminescence

Deep ultraviolet

Ions

Terbium

Tissue optics

Microscopy

Pathology

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