Effect of formalin fixing on chromophore saliency maps derived from multi-spectral macropathology skin images

Eleni Aloupogianni; Hiroyuki Suzuki; Takaya Ichimura; Atsushi Sasaki; Hiroto Yanagisawa; Tetsuya Tsuchida; Masahiro Ishikawa; Naoki Kobayashi; Takashi Obi

doi:10.1117/12.2581926

15 February 2021 Effect of formalin fixing on chromophore saliency maps derived from multi-spectral macropathology skin images

Eleni Aloupogianni, Hiroyuki Suzuki, Takaya Ichimura, Atsushi Sasaki, Hiroto Yanagisawa, Tetsuya Tsuchida, Masahiro Ishikawa, Naoki Kobayashi, Takashi Obi

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Proceedings Volume 11603, Medical Imaging 2021: Digital Pathology; 116030W (2021) https://doi.org/10.1117/12.2581926
Event: SPIE Medical Imaging, 2021, Online Only

Conference Poster

Abstract

Evaluation of tissue margins and hemodynamics is necessary during macropathology of skin lesions. This study aims to produce saliency maps of skin chromophores from ex-vivo specimens and observe the effect of formalin fixation on the maps. We used a multi-spectral imaging system with narrow-band illumination to capture various skin lesions. Saliency maps were produced with three different methods adapted from the literature by utilizing spectral absorption and absorption slope. Saliency maps derived from fixed and unfixed tissue were registered and subsequently compared in terms of correlation and histogram similarity. Preliminary results show high dissimilarity between maps of fixed and unfixed tissue, highlighting the influence of formalin fixing on hemodynamics, while relative distribution of melanin remained mostly unaffected.

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Eleni Aloupogianni, Hiroyuki Suzuki, Takaya Ichimura, Atsushi Sasaki, Hiroto Yanagisawa, Tetsuya Tsuchida, Masahiro Ishikawa, Naoki Kobayashi, and Takashi Obi "Effect of formalin fixing on chromophore saliency maps derived from multi-spectral macropathology skin images", Proc. SPIE 11603, Medical Imaging 2021: Digital Pathology, 116030W (15 February 2021); https://doi.org/10.1117/12.2581926

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