Retinal diseases such as diabetic retinopathy and glaucoma are leading cause of blindness in the world. Assessing biomechanical properties of retina is very crucial since it is constantly under stress due to the vitreous humor and eye movements. Characterizing biomechanical properties of retina noninvasively is a challenge due to its location inside the eye-globe, fragility, and thin geometry. Brillouin microscopy is a noninvasive, all optical imaging technique to qualitatively map the biomechanical properties of tissues. In this work, we mapped the layer by layer distribution of biomechanical properties of retinas using Brillouin microscopy. We found that the nuclear layer was stiffer compared to other layers. Furthermore, we observed fixing the retinas with paraformaldehyde increased the retinal stiffness compared to the fresh retinas.
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