Ultrafast two-photon volumetric scanning microscopy

Chien-Sheng Wang; Yu-Hsuan Tsai; Po-Yuan Wang; Chih-Wei Liu; Chia-Fu Chou; Vijay Raj Singh; Peter T. C. So; Shi-Wei Chu

doi:10.1117/12.2582693

5 March 2021 Ultrafast two-photon volumetric scanning microscopy

Chien-Sheng Wang, Yu-Hsuan Tsai, Po-Yuan Wang, Chih-Wei Liu, Chia-Fu Chou, Vijay Raj Singh, Peter T. C. So, Shi-Wei Chu

Proceedings Volume 11654, High-Speed Biomedical Imaging and Spectroscopy VI; 116540F (2021) https://doi.org/10.1117/12.2582693
Event: SPIE BiOS, 2021, Online Only

Abstract

Here we achieved record-high >500 volumes/second two-photon imaging by improving lateral and axial scanning speed via 32-channel multifocal excitation/detection, and a tunable acoustic gradient-index lens, respectively. We developed a deconvolution process to reduce scattering-induced crosstalk in multifocal detection scheme, thus enabling whole brain imaging of Drosophila with millisecond and micrometer spatiotemporal resolution. Potential applications toward brain science include studying millisecond dynamics in a neuronal network, and resolving 3D microfluidics in blood vessels.

Conference Presentation

Citation Download Citation

Chien-Sheng Wang, Yu-Hsuan Tsai, Po-Yuan Wang, Chih-Wei Liu, Chia-Fu Chou, Vijay Raj Singh, Peter T. C. So, and Shi-Wei Chu "Ultrafast two-photon volumetric scanning microscopy", Proc. SPIE 11654, High-Speed Biomedical Imaging and Spectroscopy VI, 116540F (5 March 2021); https://doi.org/10.1117/12.2582693

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