Deep tissue light sheet imaging with structured illumination

Leilei Peng

doi:10.1117/12.2579295

5 March 2021 Deep tissue light sheet imaging with structured illumination

Leilei Peng

Proceedings Volume 11657, Biomedical Applications of Light Scattering XI; 116570B (2021) https://doi.org/10.1117/12.2579295
Event: SPIE BiOS, 2021, Online Only

Abstract

Traditional optical imaging systems are designed with the assumption that light travels along straight paths inside the sample. Light scattering in biological tissue leads to high background and blurry images in traditional imaging methods. We report technique advancements in structured illumination with light sheet imaging (SIM-LS) in deep tissue. SIM-LS is capable of removing image background generated by scattering and restoring optical resolution in deep tissue imaging. With two-photon Bessel beam excitation, SIM-LS supports up to 500 micron wide field of view 3D imaging in embryos and brain tissue at subcellular resolution.

Conference Presentation

Citation Download Citation

Leilei Peng "Deep tissue light sheet imaging with structured illumination", Proc. SPIE 11657, Biomedical Applications of Light Scattering XI, 116570B (5 March 2021); https://doi.org/10.1117/12.2579295

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