Spatiotemporal quantification of actin dynamics by non-bleaching nanoscale imaging

Xintao Zhao; Di Zu; Guangjie Cui; Yunbo Liu; Ancheng Da; John Gregg; Somin Eunice Lee

doi:10.1117/12.2578378

9 March 2021 Spatiotemporal quantification of actin dynamics by non-bleaching nanoscale imaging

Xintao Zhao, Di Zu, Guangjie Cui, Yunbo Liu, Ancheng Da, John Gregg, Somin Eunice Lee

Proceedings Volume 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII; 1165807 (2021) https://doi.org/10.1117/12.2578378
Event: SPIE BiOS, 2021, Online Only

Abstract

Actin dynamics plays an important role in cell activities and drives a large range of cellular process such as cell division and cell motility. To visualize the actin dynamics, we introduce non-bleaching nanoscale imaging to capture actin dynamics over long time scales. Here, we demonstrate the high resolution quantification of actin dynamics by non-bleaching nanoscale imaging for the purpose of revealing resulting mechanical properties. Through this method, we visualized and quantified actin twisting dynamics through disassembly process by actin binding proteins.

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Citation Download Citation

Xintao Zhao, Di Zu, Guangjie Cui, Yunbo Liu, Ancheng Da, John Gregg, and Somin Eunice Lee "Spatiotemporal quantification of actin dynamics by non-bleaching nanoscale imaging", Proc. SPIE 11658, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVIII, 1165807 (9 March 2021); https://doi.org/10.1117/12.2578378

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