A holographic method for generating structured illumination of structured illumination microscopy

Xianlin Song

doi:10.1117/12.2581309

18 April 2021 A holographic method for generating structured illumination of structured illumination microscopy

Xianlin Song

Proceedings Volume 11774, Holography: Advances and Modern Trends VII; 117740E (2021) https://doi.org/10.1117/12.2581309
Event: SPIE Optics + Optoelectronics, 2021, Online Only

Abstract

We use a holographic method to generate structured illumination which of spatial frequency and phase can be easily modulated. First, a black and white stripe with a certain spatial frequency is generated according to the period and phase of structured illumination, the black area is 0 and the white area is 1. The spatial frequency of the black and white fringe is determined by calculating the spatial frequency of the structured illumination to be finally generated and the magnification of the optical system. A prism phase that can cause lateral movement is applied to the black part of the black and white stripes, so that the light that incident on the black area tilts from the optical axis to the first order of diffraction. While, the light that incident on other parts is not modulated and does not move laterally, and continues traveling along with optical axis. In this way, the tilted beam is bright and dark, and the diaphragm is used to block the 0- order diffracted light and retain the diffracted first-order light, thereby obtaining cosine structured light. Theories and experiments verify the effectiveness of the method. The method to generate cosine structured illumination is simple, fast and accurate, and the spatial frequency and phase can be conveniently adjusted, which is very helpful for the research of structured illumination microscope.

Conference Presentation

Citation Download Citation

Xianlin Song "A holographic method for generating structured illumination of structured illumination microscopy", Proc. SPIE 11774, Holography: Advances and Modern Trends VII, 117740E (18 April 2021); https://doi.org/10.1117/12.2581309

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