Label-free imaging of cell nucleus dynamics by coherence brightfield (COBRI) microscopy

Yi-Teng Hsiao; Chia-Ni Tsai; Chia-Lung Hsieh

doi:10.1117/12.2615857

27 October 2021 Label-free imaging of cell nucleus dynamics by coherence brightfield (COBRI) microscopy

Yi-Teng Hsiao, Chia-Ni Tsai, Chia-Lung Hsieh

Proceedings Volume 11925, Biomedical Imaging and Sensing Conference 2021; 1192519 (2021) https://doi.org/10.1117/12.2615857
Event: SPIE Technologies and Applications of Structured Light, 2021, Online, Japan

Abstract

Current optical microscope imaging heavily relies on fluorescence-based detection because of its high detection sensitivity and molecular specificity through labeling. However, the photophysics and photochemistry of the fluorophores, e.g., photobleaching, blinking and saturation, have limited its applicability to long-term observation and quantitative imaging. Label-free interference microscopy potentially overcomes the challenges aforementioned by measuring the scattered field of the sample quantitatively, but these approaches usually compromise on the sensitivity and molecular specificity of imaging. Here, we demonstrate coherent brightfield (COBRI) microscopy, a highly sensitive scatteringbased interference microscopy, to capture the dynamic linear scattering signal of live cell nucleus. We demonstrate a strategy to reconstruct the nuclear organization from the time-lapse COBRI imaging. Our method offers the opportunity to investigate the cell nuclear dynamics at the unprecedented spatial and temporal resolutions.

Citation Download Citation

Yi-Teng Hsiao, Chia-Ni Tsai, and Chia-Lung Hsieh "Label-free imaging of cell nucleus dynamics by coherence brightfield (COBRI) microscopy", Proc. SPIE 11925, Biomedical Imaging and Sensing Conference 2021, 1192519 (27 October 2021); https://doi.org/10.1117/12.2615857

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