Hydraulic force spectroscopy: an interferometry-based approach to micropipette aspiration for mechanobiology studies at the nanoscale

Massimiliano Berardi; Eline Bogers; Kevin Bielawski; Imran B. Acka

doi:10.1117/12.2606184

2 March 2022 Hydraulic force spectroscopy: an interferometry-based approach to micropipette aspiration for mechanobiology studies at the nanoscale

Massimiliano Berardi, Eline Bogers, Kevin Bielawski, Imran B. Acka

Author Affiliations +

Proceedings Volume 11962, Optical Elastography and Tissue Biomechanics VIV; 1196205 (2022) https://doi.org/10.1117/12.2606184
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

We present a new approach to Micropipette Aspiration (MPA), a pioneering method in mechanobiology, that introduces an all-optical readout to retrieve both applied stress and material response. Our technique, i.e. Hydraulic Force Spectroscopy, expands on the current MPA experimental possibilities, allowing for frequency-dependent complex moduli measurements of soft suspended bodies over a large bandwidth, with nanometric resolution. This is achieved by oscillating the pressure on the sample by tens of Pascals with a multifrequency signal, by means of a fast pump. Our goal is to use this technique to define new label-free biomarkers for different applications, e.g. embryo viability control.

Conference Presentation

Citation Download Citation

Massimiliano Berardi, Eline Bogers, Kevin Bielawski, and Imran B. Acka "Hydraulic force spectroscopy: an interferometry-based approach to micropipette aspiration for mechanobiology studies at the nanoscale", Proc. SPIE 11962, Optical Elastography and Tissue Biomechanics VIV, 1196205 (2 March 2022); https://doi.org/10.1117/12.2606184

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