A self-validating fiber-optic surface plasmon resonance (FO-SPR) sensor system

Aditi Chopra; Girish C. Mohanta; Shwinky Bhatti; Sudipta Sarkar

doi:10.1117/12.2607237

3 March 2022 A self-validating fiber-optic surface plasmon resonance (FO-SPR) sensor system

Aditi Chopra, Girish C. Mohanta, Shwinky Bhatti, Sudipta Sarkar

Proceedings Volume 11978, Plasmonics in Biology and Medicine XIX; 1197806 (2022) https://doi.org/10.1117/12.2607237
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Surface plasmon resonance (SPR) based sensors have emerged as an excellent analytical technique known for their ultra-high sensitivity capable of detecting even minute refractive index (RI) changes of the surrounding during biomolecular interactions. However, due to their exceptional sensitivity towards RI changes, they are also susceptible to variations to reaction parameters such as temperature, concentration and non-specific adsorption which contributes to its inherent non-specificity. To circumvent these problems, researchers have employed various downstream techniques like chromatographic separations linked with Mass spectroscopy (MS) to ascertain sensor specificity. In an attempt to resolve this issue, we have exploited Surface-enhanced Raman Spectroscopy (SERS) as a secondary tool to validate the specificity of SPR signals. For this purpose, we have utilized a fiber-optic SPR system and gold nanoparticles to create a “metal film-molecule- metal nanoparticle” configuration and have shown that the resultant enhancement in the Raman signal of the molecule can be utilized as a self-validating tool. Accordingly, we have functionalized our FO-SPR with the bifunctional Raman active molecules; 4- aminothiolphenol (4-ATP), which could interact with carboxylated gold nanoparticles to create a “metal film-moleculemetal nanoparticle” configuration. The successful binding of the gold nanoparticles was reflected in ~19 nm SPR shift along with the simultaneous increase in the Raman signals of the 4-ATP molecules sandwiched in-between metal film and gold nanoparticles.

Conference Presentation

Citation Download Citation

Aditi Chopra, Girish C. Mohanta, Shwinky Bhatti, and Sudipta Sarkar "A self-validating fiber-optic surface plasmon resonance (FO-SPR) sensor system", Proc. SPIE 11978, Plasmonics in Biology and Medicine XIX, 1197806 (3 March 2022); https://doi.org/10.1117/12.2607237

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