Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view

Chenting Lai; Bernhard Messerschmidt; Sven Flämig; Karl Reichwald; Hyeonsoo Bae; Matteo Calvarese; Tobias Meyer; Michael Schmitt; Franziska Hoffmann; Orlando Guntinas-Lichius; Jürgen Popp

doi:10.1117/12.2621692

27 May 2022 Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view

Chenting Lai, Bernhard Messerschmidt, Sven Flämig, Karl Reichwald, Hyeonsoo Bae, Matteo Calvarese, Tobias Meyer, Michael Schmitt, Franziska Hoffmann, Orlando Guntinas-Lichius, Jürgen Popp

Author Affiliations +

Proceedings Volume 12144, Biomedical Spectroscopy, Microscopy, and Imaging II; 1214408 (2022) https://doi.org/10.1117/12.2621692
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

Here, we report a new handheld endoscopic system for nonlinear multimodal imaging of the head and neck region. It has a long rigid endomicroscopic probe with two versions (0° and 45° bended tip), connecting with a compact scan-head of approx. 10×12×6 cm³ size. The rigid probe is 6 mm in diameter and 24 cm long and allows diffraction-limited multiphoton imaging of tissue with at least 430 μm field of view and sub-micron resolution. The signals of Coherent anti-Stokes Raman Scattering (CARS), second harmonic generation (SHG), and two-photon excited fluorescence (TPEF) are collected by a non-descanned detection path in the scan-head, and the fluorescence of Indocyanine green (ICG) labeled lesions is detected by a confocal descanned configuration. Furthermore, this system is capable of guiding high-power femtosecond laser pulses for tissue ablation without the risk of damaging optical glass components.

Conference Presentation

Citation Download Citation

Chenting Lai, Bernhard Messerschmidt, Sven Flämig, Karl Reichwald, Hyeonsoo Bae, Matteo Calvarese, Tobias Meyer, Michael Schmitt, Franziska Hoffmann, Orlando Guntinas-Lichius, and Jürgen Popp "Multimodal handheld endomicroscopic system designed for in vivo laser ablation and nonlinear imaging with a large field of view", Proc. SPIE 12144, Biomedical Spectroscopy, Microscopy, and Imaging II, 1214408 (27 May 2022); https://doi.org/10.1117/12.2621692

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available