Study on liquid-liquid phase separation based on optical tweezers

Shuai Li; Haiqing Liu; Huizhu Hu

doi:10.1117/12.2624587

27 March 2022 Study on liquid-liquid phase separation based on optical tweezers

Shuai Li, Haiqing Liu, Huizhu Hu

Proceedings Volume 12169, Eighth Symposium on Novel Photoelectronic Detection Technology and Applications; 121695W (2022) https://doi.org/10.1117/12.2624587
Event: Eighth Symposium on Novel Photoelectronic Detection Technology and Applications, 2021, Kunming, China

Abstract

An optical method for achieving liquid-liquid phase separation (LLPS) was reported. By using optical tweezers (OT) technique, the highly focused laser trap can produce a microdroplet in the mixture of inorganic phosphate buffered saline (PBS) and organic solvent isopropanol. The Raman test method revealed that the phosphate microdroplet was the product of LLPS. The controllable generation of microdroplets can be achieved by adjusting the proportion of two components in the mixture (1:3 to 1:40). With advantages in precise manipulation and controllable growth of single microdroplet, such an OT-based LLPS method promotes potential innovation ideas in the fields of disease treatment, intracellular structures, and droplet microfluidics.

Citation Download Citation

Shuai Li, Haiqing Liu, and Huizhu Hu "Study on liquid-liquid phase separation based on optical tweezers", Proc. SPIE 12169, Eighth Symposium on Novel Photoelectronic Detection Technology and Applications, 121695W (27 March 2022); https://doi.org/10.1117/12.2624587

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