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3 October 2022 Laser-induced photolysis leads to a Ca2+ transient in surrounding astrocytes which is dependent on internal stores
Veronica Gomez Godinez, Huayan Li, Yixuan Kuang, Linda Shi, Michael W. Berns
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Proceedings Volume 12198, Optical Trapping and Optical Micromanipulation XIX; 121980E (2022) https://doi.org/10.1117/12.2632869
Event: SPIE Nanoscience + Engineering, 2022, San Diego, California, United States
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Abstract
Astrocytes in the brain migrate to sites of injury where they can take up damaging molecules extruded from injured cells to protect neurons. The astrocytic response to cell death is critical to our understanding of ways to mitigate secondary injury from a traumatic brain injury(TBI). We previously showed that a laser could be used to induce a single cell death (photolysis) in order to monitor the surrounding astrocytic response. We found that photolysis leads to a calcium transient in surrounding astrocytes. Here we show that cells treated with the internal calcium chelator BAPTA-AM do not exhibit a transient. Similarly, cells whose endoplasmic reticulum (ER) has been depleted through blocking of the SERCA pump do not show a calcium increase. Cells treated with EGTA to chelate external calcium showed no statistical significance when compared to cells in regular medium with calcium. Therefore, it is concluded that the ER stores are largely responsible for the cytosolic calcium transient.
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Veronica Gomez Godinez, Huayan Li, Yixuan Kuang, Linda Shi, and Michael W. Berns "Laser-induced photolysis leads to a Ca2+ transient in surrounding astrocytes which is dependent on internal stores", Proc. SPIE 12198, Optical Trapping and Optical Micromanipulation XIX, 121980E (3 October 2022); https://doi.org/10.1117/12.2632869
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KEYWORDS
Calcium
Photolysis
Cell death
Brain
Injuries
Traumatic brain injury
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