High-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions

Giacomo Mazzamuto; Ludovico Silvestri; Giuseppe Sancataldo; Vladislav Gavryusev; Marina Scardigli; Irene Costantini; Francesco S. Pavone

doi:10.1117/12.2650374

16 May 2023 High-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions

Giacomo Mazzamuto, Ludovico Silvestri, Giuseppe Sancataldo, Vladislav Gavryusev, Marina Scardigli, Irene Costantini, Francesco S. Pavone

Author Affiliations +

Proceedings Volume 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX; 1238504 (2023) https://doi.org/10.1117/12.2650374
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

High-speed imaging with light-sheet fluorescence microscopy poses several challenges throughout the whole pipeline, from data acquisition in the lab to image stitching and post-processing. Here we present our custom hardware and software solutions that allow us to map large biological samples at the cellular level, e.g. large portions of human brain cortex. Our custom optical setup—a dual-view, inverted, light-sheet microscope—is capable of simultaneous two-color acquisition at a data rate of 1 GB/s. Our open source tools include the instrument’s data acquisition and control software and also cover volumetric image stitching and post-processing.

Conference Presentation

Citation Download Citation

Giacomo Mazzamuto, Ludovico Silvestri, Giuseppe Sancataldo, Vladislav Gavryusev, Marina Scardigli, Irene Costantini, and Francesco S. Pavone "High-speed light-sheet microscopy imaging and data post-processing using custom hardware and software solutions", Proc. SPIE 12385, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXX, 1238504 (16 May 2023); https://doi.org/10.1117/12.2650374

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