Two-photon speckle excitation fluorescence imaging using non-negative matrix factorization

T. Monno; X. Quan; K. Ohta; N. Yoneda; O. Matoba

doi:10.1117/12.3007935

20 September 2023 Two-photon speckle excitation fluorescence imaging using non-negative matrix factorization

T. Monno, X. Quan, K. Ohta, N. Yoneda, O. Matoba

Proceedings Volume 12608, Biomedical Imaging and Sensing Conference; 126081B (2023) https://doi.org/10.1117/12.3007935
Event: Optics and Photonics International Congress, 2023, Yokohama, Japan

Abstract

The objective of this study was to establish a scattering imaging method that uses non-negative matrix factorization (NMF) to remove the effects of scattering from images taken of scattered light and to recover the original object information. In addition, fluorescence imaging by two-photon excitation using random pattern illumination was newly tackled. Fluorescent beads are emitted by two-photon excitation with random pattern illumination, and the scattered light is captured and reconstruction is implemented using memory effect, non-negative matrix factorization and correlation calculation.

Citation Download Citation

T. Monno, X. Quan, K. Ohta, N. Yoneda, and O. Matoba "Two-photon speckle excitation fluorescence imaging using non-negative matrix factorization", Proc. SPIE 12608, Biomedical Imaging and Sensing Conference, 126081B (20 September 2023); https://doi.org/10.1117/12.3007935

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